Postdoctoral Research Assistant - Proteases Reshaping the Secretome

University of Oxford - Department of Plant Sciences

South Parks Road, Oxford

The Department of Plant Sciences is seeking to recruit a Postdoctoral Research Assistant. This is a 1-year contract funded by the ERC. This grant is held by Professor van der Hoorn and the work is to be conducted in his lab in the Department of Plant Sciences, South Parks Road, Oxford. The research topic is ‘Proteases reshaping the secretome’.

The responsibilities/duties of the postholder will be: design, clone, and transform small guide RNA for genome editing in N. benthamiana; select homozygous plants carrying the desired mutation(s); isolate secreted proteomes and analyse this by various proteomic techniques; infection assays with Pseudomonas syringae on protease depleted plants; monitor recombinant protein production upon agroinfiltration; work with other team members within the GreenProteases research program.

The postholder should: have or be expected to obtain a PhD or equivalent and publication record in a relevant area; Extensive expertise in protein work (western blot, 2D gels, pull downs, etc.). Documented experience with molecular cloning. Experience with Pseudomonas syringae infections. Desirable expertise with clean bench work for plant tissue culture. Experience with activity-based protein profiling (ABPP). Experience in generating and evaluating MS data. Experience with working in a team.

Informal enquiries should be directed to Professor van der Hoorn at:

The closing date for applications is 12.00 noon on Friday 16 December 2016.

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South East England