|Salary:||£27,116 to £40,927|
|Placed On:||14th October 2021|
|Closes:||15th November 2021|
The Wellcome - Medical Research Council Cambridge Stem Cell Institute is an international centre of excellence for stem cell research and regenerative medicine. Scientists in the Institute collaborate to advance our knowledge of various stem cell types and to perform pioneering work in translational research areas, providing the foundation for new medical treatments (https://www.stemcells.cam.ac.uk/).
The laboratories of Dr Kevin Chalut (https://www.stemcells.cam.ac.uk/people/pi/chalut, https://scholar.google.com/citations?hl=en&user=J0m7YbsAAAAJ) and Dr Srinjan Basu (https://www.stemcells.cam.ac.uk/people/pi/basu,https://scholar.google.com/citations?hl=en&tzom=-60&user=Ds1P8F0AAAAJ) develop and implement multidisciplinary approaches to understand how pluripotent stem cells respond to physical forces and how this response influences cell fate (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385134/; https://www.biorxiv.org/content/10.1101/2020.04.03.003178v2; https://www.sciencedirect.com/science/article/pii/S1934590920305348; https://www.nature.com/articles/s41586-019-1484-9). Regardless of the origin of the force, the cytoskeleton propagates these stresses to the nucleus through a process called nuclear mechanotransduction (https://www.sciencedirect.com/science/article/pii/S0955067420300685?via%3Dihub). Nuclear envelope proteins play a key role in this process but how they influence nuclear processes during pluripotent cell differentiation remain poorly understood. We are therefore looking for a dedicated post-doctoral researcher to determine how these proteins respond to physical forces, how they influence chromatin architecture and how they influence cell fate. The post holder will use a range of state-of-the-art techniques including super-resolution imaging of nuclear proteins, next-generation sequencing (chromosome conformation capture, CUT&Tag/ChIP-seq, RNAseq) including singl-cell Hi-C (https://www.nature.com/articles/nprot.2018.017/boxes/bx5) and cell stretching assays (https://royalsocietypublishing.org/doi/full/10.1098/rsob.180203). They will work with a bioinformatician to analyse this data and with a lab manager who will help generate appropriate cell lines.
Successful applicants should have a PhD* (postgraduate/MSc degree for the research assistant role) in the field of cell/developmental biology, mechanobiology or chromatin biology or be within 6 months of the completion of their PhD. Additional technical expertise that would be useful for this position include pluripotent stem cell culture/differentiation, super-resolution imaging, microfluidics and/or next-generation sequencing. It is essential that the post-holder is organised, able to work collaboratively as a member of a team and can communicate effectively and clearly.
*Appointment at Research Associate level is dependent on having a PhD or an equivalent research doctorate. Those who have submitted but not yet received their PhD will be appointed at Research Assistant level, which will be amended to Research Associate once PhD has been awarded.
Start date is flexible but ideally January 2021.
Fixed-term: The funds for this post are available for 24 months in the first instance.
Once an offer of employment has been accepted, the successful candidate will be required to undergo a security check.
To apply online for this vacancy and to view further information about the role, please visit :
The closing date for all applications is 15 November 2021 with interviews in the last week of November.
Please quote reference PS28490 on your application and in any correspondence about this vacancy.
The University actively supports equality, diversity and inclusion and encourages applications from all sections of society.
The University has a responsibility to ensure that all employees are eligible to live and work in the UK.
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