|Salary:||£40,125 with benefits, subject to skills and experience|
|Placed On:||9th August 2022|
|Closes:||8th October 2022|
The Research Group
The Protein Biogenesis lab (https://www.crick.ac.uk/research/labs/david-balchin) at the Francis Crick institute is looking for a postdoc with expertise in single-particle Cryo-EM, to help us understand how molecular chaperones collaborate with the ribosome to catalyse protein folding.
The Crick was formed in 2015, and in 2016 it moved into a new state-of-the-art building in central London which brings together 1500 scientists and support staff working collaboratively across disciplines, making it the biggest biomedical research facility under in one building in Europe. In addition to excellent facilities for Cryo-EM, the institute houses cutting-edge platforms for structural biology, biophysics and proteomics, amongst many others.
Protein folding begins during translation at the ribosome and is assisted by diverse molecular chaperones that engage the nascent polypeptide before its synthesis is complete. Although cotranslational folding and chaperone action are often critical to efficient protein biogenesis, the underlying mechanisms are poorly understood, especially from a structural perspective.
Our group has recently optimized methods for preparing highly homogeneous and stable protein biogenesis intermediates from bacterial and human cells. These huge complexes represent snapshots of authentic protein folding reactions, and contain the ribosome, partially folded nascent protein and stably-bound chaperones. In preliminary work we have studied these complexes using state-of-the-art biochemical and proteomic approaches, allowing us to define the contribution of the bacterial ribosome and molecular chaperones to protein folding.
The successful applicant must have expertise in single-particle cryo-electron microscopy (sample preparation to data analysis).
The Francis Crick Institute is a biomedical discovery institute dedicated to understanding the fundamental biology underlying health and disease. Its work is helping to understand why disease develops and to translate discoveries into new ways to prevent, diagnose and treat illnesses such as cancer, heart disease, stroke, infections, and neurodegenerative diseases.
An independent organisation, its founding partners are the Medical Research Council (MRC), Cancer Research UK, Wellcome, UCL (University College London), Imperial College London and King’s College London.
The Crick was formed in 2015, and in 2016 it moved into a new state-of-the-art building in central London which brings together 1500 scientists and support staff working collaboratively across disciplines, making it the biggest biomedical research facility under in one building in Europe.
The Francis Crick Institute will be world-class with a strong national role. Its distinctive vision for excellence includes commitments to collaboration; developing emerging talent and exporting it the rest of the UK; public engagement; and helping turn discoveries into treatments as quickly as possible to improve lives and strengthen the economy.
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