Location: | Durham |
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Salary: | £37,099 to £44,263 per annum |
Hours: | Full Time |
Contract Type: | Fixed-Term/Contract |
Placed On: | 12th August 2024 |
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Closes: | 6th September 2024 |
Job Ref: | 24001501 |
The Role
Applications are invited for a Postdoctoral Research Associate in Microbial Metal Sensing. The research project is lead by Dr. Peter Chivers, as part of the BBSRC-funded ELEMENTAL Engineering Biology Hub (). The project will focus on engineering bacterial strains with altered cytosolic metal availabilities (see for a recent review), with applications ranging from improved metallation of heterologously expressed metalloenzymes and metallocofactors to improved metal tolerance in strains used in the circular economy of metals. The work at Durham will link directly with Hub partner activities at the University of Manchester and Quadram Institute.
The project will involve a range of experimental methods including assays of native and engineering bacterial strains for growth, gene expression (qPCR), and metal content (ICP-MS). Protein overexpression and purification (using untagged constructs) will be used to enable biophysical analysis of metal-protein interactions (e.g. affinity determination by competition assays). Regular joint meetings between academic partners will be a key part of meeting project goals and outcomes, along with presentations at annual Hub research meetings.
Key responsibilities:
This post is fixed term for 24 months from 1st November 2024 to 31st October 2026 as part of a time-limited funded research project. There is a possibility of extension for a further 24 months to 31st October 2028 pending initial project outcomes with input from the Hub Management Team and Strategic Advisory Board.
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