Advanced Research Assistant - 1 year Fixed Term Contract

Wellcome Trust Sanger Institute - Biological Sciences

The post holder's main responsibilities will be execution of day-to-day workflows of projects for the generation of targeting vectors, generation of CRISPR/Cas 9 reagents and the generation and validation of quality assured targeted ES cells. Key responsibilities include execution and timely delivery of these resources, accurate record keeping, project troubleshooting and an ability to work across multiple projects. In addition, the group also has a commitment to develop and implement approaches that facilitate the insertion of complex alleles directly into the mouse zygote with a greater efficiency than current methods permit.

Previous experience of mammalian cell culture, especially mouse ES cell culture is a prerequisite for this role. The candidate would also be expected to have a broad knowledge of molecular biology techniques, especially relating to nucleic acid extraction, molecular cloning and PCR based genotyping techniques. An understanding of genome browsers, conventional gene targeting and the more recent CRISPR/Cas9 genome engineering would be advantageous. Good communication/record keeping skills and the ability to independently handle complex workflows from project inception to delivery would be key.

Some weekend cell culture is anticipated in this role.

Essential Skills

  • Highly experienced in mammalian cell culture, especially routine mouse ES cells culturing.
  • An understanding and practical experience in molecular biology strategies and techniques, including some of the following, molecular cloning, construct generation (via conventional cloning, Gibson assembly and recombineering), PCR, restriction enzyme analysis and sequencing.
  • Isolation and purification of nucleic acid, particularly RNA based methodologies for the generation of CRISPR reagents.
  • Capable of performing/tracking a variety of challenging projects simultaneously.
  • Ability to work as part of a team and also independently once appropriate training and guidance has been provided.
  • Good communication skills to allow accurate information to be exchanged.
  • Science degree in biological sciences or extensive, proven practical experience.
  • Ability to schedule and deliver simultaneous projects within agreed deadlines.
  • Good computer skills.

Ideal Skills

  • Experience of transfection and electroporation technologies.
  • Practical experience and theoretical understanding of gene targeting strategies.
  • Practical experience and theoretical understanding of genotyping techniques, including qPCR and long range PCR and data interpretation thereof.
  • Practical experience in understanding gene structures on standard genome browsers.
  • Ability to test and implement new approaches and techniques including troubleshooting. 

Other information

The Sanger Institute is a charitably funded research centre focused on understanding the role of genetics in health and disease. Our goal is to provide results that can be translated into diagnostics, treatments or therapies that reduce global health burdens.

The Institute is located near Cambridge, UK on the stunning Wellcome Genome Campus. This growing and dynamic site is the British hub of genomic science. Its vision is to inspire a range of audiences both on the Campus and beyond it, and make the world of genomics open and accessible to all.

Our Benefits include: Defined Contribution Pension Scheme, Group Income Protection, Healthcare scheme, Childcare Vouchers, Workplace Nursery and 25 days Annual Leave, increasing by one day per year up to a maximum of 30, plus Bank Holidays. We also have a gym, two cafes, dining facilities, and a free campus bus service.

Wellcome Trust Sanger Institute welcomes applications irrespective of age, disability, gender, gender identity, sexual orientation, race, religion or belief, or marital or civil partnership status.

Please include a covering letter and CV with your application.

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South East England