Understanding variation of iron-sulfur cluster type and coordination within a single regulatory protein framework (LEBRUN_U17DTP2)

University of East Anglia - School of Chemistry

Start Date:  1st October 2017

No. of positions available: 1

Supervisor: Prof. Nick Le Brun 

Project description: 
The Rrf2 family of regulators is widespread amongst bacteria and control some of the most important cellular pathways, including iron metabolism, iron-sulfur biogenesis and the response to oxidative and nitrosative stress.  Many Rrf2 family regulators bind an iron-sulfur cluster, and this is the key sensory module of the regulator.  It turns out that although Rrf2 proteins are very similar to one another, the type of cluster they bind, and the way that they do it, varies from one family protein to another.  This project is focused on determining cluster type and coordination and understanding the important factors that control these characteristics. The consequences of altering cluster coordinating residues on cluster type and functionality will also be investigated. The successful applicant will receive training in a wide range of techniques during the project, including protein purification, anaerobic protein handling, iron-sulfur cluster chemistry, protein-DNA interactions, spectroscopy, mass spectrometry and X-ray crystallography.  The project will be based mainly in the Le Brun lab (School of Chemistry at UEA), with interactions with the Hutchings and Todd Labs (School of Biological Sciences at UEA) and the Fontecilla-Camps Lab (Grenoble, France).

Person specification:  Minimum entry 2:1

Funding notes: Full Studentships cover a stipend (RCUK rate: £14,296pa – 2016/7), research costs and tuition fees at UK/EU rate, and are available to UK and EU students who meet the UK residency requirements.

Students from EU countries who do not meet the UK residency requirements may be eligible for a fees-only award. Students in receipt of a fees-only award will be eligible for a maintenance stipend awarded by the NRPDTP Bioscience Doctoral Scholarships, which when combined will equal a full studentship. To be eligible students must meet the EU residency requirements.

Details on eligibility for funding on the BBSRC website: www.bbsrc.ac.uk/web/FILES/Guidelines/studentship_eligibility.pdf

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