Research Associate

King's College London - Centre for Stem Cells and Regenerative Medicine

The salary will be paid at Grade 6, £33,518 per annum, plus £2,923 per annum London Allowance.

The contract is until 22 April 2019

This is a Full-time – 100% full time equivalent

Stem cells are one of the fundamental underpinnings of tissue biology. They allow myriad tissues to be replenished by fresh cells throughout life. To preserve the regenerative capacity of the tissue, it is essential to maintain the number of stem cells. One mechanism that balances both tasks is asymmetric cell division (ACD). In ACD, each stem cell divides to generate one daughter with a stem-cell fate (self-renewal) and one daughter that differentiates.

Wnt signals are key proteins that regulate stem cell maintenance. In vivo, Wnt proteins are often secreted locally and presented to only one side of the responsive cell. Our laboratory has succeeded in covalently immobilising biologically active Wnt proteins to beads, thus revolutionising the process of specifically targeting Wnt proteins to individual cells and tissues. By using this novel tool and advanced live imaging, we showed that Wnt-beads can induce oriented ACD of embryonic stem cells (ESCs). Accordingly, ACD produces one Wnt-proximal ESC and one Wnt-distal epiblast-like stem cell (for more details see Habib et al 2013 Science Mar 22;339(6126):1445-8 and visit our website: (www.habiblab.org).

The aim of the proposed project is to dissect the epigenetic modifications during Wnt-mediated ACD. To that end the Research Associate (RA) will apply principles from genetics biochemistry, and stem cell biology in conjunction with advanced imaging techniques to study the dynamics of epigenetic modifications. Specifically, the RA will use real-time 3D microscopy to follow the dynamics of components of the epigenetic machinery (e.g. Dnmt3 proteins) during ACD. They will focus on the link between the epigenetic machineries and modifications, cell fate choice and components of the Wnt pathway that have been proposed to be involved in regulating ACD.

The successful candidate is expected to:

1) Have a good knowledge in mouse embryonic stem cells and epigenetics.

2) A PhD in Biological Sciences

3) Expertise in cell live imaging and image analysis.

4) Excellent skills in molecular biology techniques including gene cloning.

How to apply: In addition to your application, please submit your CV (maximum three sides of A4) and a covering letter.  In your covering letter (maximum one side of A4), please summarise your previous research experience(s) and explain why you are interested in our laboratory. Also please write a brief preliminary proposal (one side of A4) of experiments that you think will be suitable for this project. Please note that applications submitted without a proposal will not be considered.

The selection process will include scientific proposal to be submitted with the application, competency based questions, a presentation, an assessment and a panel interview.

To apply for this role, please go to the King’s College London HireWire Job Board and register to download and submit the specified application form.

The deadline for applications is midnight on 19 October 2017.

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