Postdoctoral Training Fellow

The Francis Crick Institute

Contract: Fixed-term (4 years), full time

Short Summary

A new postdoctoral research position is available in the laboratory of Alessandro Costa at the Francis Crick Institute. We are looking for an enthusiastic researcher to study eukaryotic DNA replication using cryo-electron microscopy. The successful candidate will biochemically reconstitute key intermediates in the assembly and activation of the DNA replication machinery, with purified yeast proteins. The aim of the lab is to understand genome replication at a molecular level by i. developing a new EM-based DNA replication assay and ii. obtaining a high resolution structure of the intact, activated replisome. This research builds upon several studies published by the lab in the last four five years. Successful candidates will have access to state of the art technology, including large-scale fermentation, mass spectrometry, peptide synthesis and two Crick Titan Krios electron microscopes.

For more information about this research, please visit the external lab website: https://www.crick.ac.uk/research/a-z-researchers/researchers-a-c/alessandro-costa/

Project Scope

The new postdoc will reconstitute DNA replication intermediates for imaging by cryo-EM. The Molecular Machines lab was established in 2012 and is currently composed of seven members. The lab combines a range of expertise, including Molecular Biology & Biochemistry, Biophysics and cryo-EM. We are developing new tools to biochemically trap various stages in the assembly and activation of the eukaryotic DNA replication machinery and have tailored new image processing protocols, for the analysis of multiple protein complexes that act on the same DNA molecule. The group enjoys the highly interactive environment at the Francis Crick Institute, having established a network of collaborations with experts in genome stability maintenance and single-molecule imaging. Generous support from core-funded platforms allows access to state of the art technology including large-scale fermentation, mass spectrometry, peptide synthesis and cryo-EM.

About Us

The Francis Crick Institute is a biomedical discovery institute dedicated to understanding the fundamental biology underlying health and disease. Its work is helping to understand why disease develops and to translate discoveries into new ways to prevent, diagnose and treat illnesses such as cancer, heart disease, stroke, infections, and neurodegenerative diseases.

An independent organisation, its founding partners are the Medical Research Council (MRC), Cancer Research UK, Wellcome, UCL (University College London), Imperial College London and King’s College London.

The Crick was formed in 2015, and in 2016 it moved into a new state-of-the-art building in central London which brings together 1500 scientists and support staff working collaboratively across disciplines, making it the biggest biomedical research facility under in one building in Europe.

The Francis Crick Institute will be world-class with a strong national role. Its distinctive vision for excellence includes commitments to collaboration; developing emerging talent and exporting it the rest of the UK; public engagement; and helping turn discoveries into treatments as quickly as possible to improve lives and strengthen the economy.

The closing date for applications is 26 February 2018 at 23:30.

All offers of employment are subject to successful security screening and continuous eligibility to work in the United Kingdom.

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