Research Fellow in Cardiovascular Genetics and Development

University of Birmingham - Institute of Cancer and Genomic Sciences

The post is available from 1st April 2018 for 2 years

Applications are invited from outstanding individuals to work under the supervision of Dr Rui Monteiro, Birmingham Fellow, on a BHF funded research project to study the role of TGFb signalling in angiogenic and haemogenic endothelial cell programming. The Monteiro Lab are interested in learning how extrinsic signalling impinges on lineage fate decisions in development and how progenitors and stem cells carry out those decisions, with a particular emphasis on the Transforming Growth Factor β (TGFβ) pathway. Previous work in the lab demonstrated that TGFb1 and TGFb3 play different roles in programming haemogenic endothelium to become of blood stem cells in vivo (Monteiro et al, 2016). The Research Fellow will study the gene regulatory network that carries out the ligand-specific functions for TGFb1 and TGFb3 ligands in haemogenic and angiogenic endothelium in vivo. They will make use of several approaches, including genome editing with CRISPR/Cas9, transgenesis, fluorescence activated cell sorting and transcriptional and epigenetic profiling using zebrafish as a model.

The successful applicant will have a first degree and a PhD in developmental biology, molecular genetics, biology or in a related discipline relevant to the project. They will also have a strong background in molecular biology and previous experience with model organism and/or analysis of transcriptomic and epigenetic data.

Skills & Experience

  • A PhD degree in developmental biology, molecular genetics, biology or in a related discipline.
  • Previous experience in molecular biology such as molecular cloning, plasmid DNA purification, DNA, RNA, and protein isolation, DNA sequencing, PCR, qRT-PCR, in situ hybridization, CRISPR/Cas9 technology, etc.
  • Hold or be able to obtain a Home Office personal licence for work with zebrafish.
  • Experience with managing zebrafish colonies and with generation, identification and analysis of transgenic and/or mutant zebrafish lines, including fin clipping and genotyping.
  • Experience in zebrafish husbandry and embryo manipulation.
  • Previous experience in fluorescence activated cell sorting and microscopy, including fluorescence and confocal microscopy.
  • Ability to communicate complex information clearly.

Informal enquiries should be directed to Dr. Rui Monteiro (R.Monteiro@bham.ac.uk)

Starting salary is on Grade 7.

To download the details of this position and submit an electronic application online please click on the Apply Online button below, please quote the appropriate Job Ref in all enquiries, alternatively information can be obtained from www.hr.bham.ac.uk

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Midlands of England