Back to search results

PhD Studentship - Bioengineering Sustainable Routes to Anti-infective Agents to Combat AMR and Future Pandemics

The University of Manchester - Department of Chemistry

Qualification Type: PhD
Location: Manchester
Funding for: UK Students, EU Students
Funding amount: Not Specified
Hours: Full Time
Placed On: 18th November 2021
Closes: 21st January 2022
 

This is a BBSRC DTP studentship. Funding covers UK tuition fee and stipend only. We are able to offer a limited number of scholarships that will enable full studentships to be awarded to international applicants. These full studentships will only be awarded to exceptional candidates, due to the competitive nature of this scheme.

Supervisors: Professor Jason Micklefield, Professor Patrick Yizhi Cai & Professor Anthony Green

Project Title: Bioengineering sustainable routes to anti-infective agents to combat AMR and future pandemics

Project Description:

There is an urgent need for new anti-infective agents to combat antimicrobial resistance and to protect the global population in the event of future pandemics. The majority of drugs used to treat infectious diseases today are small molecules produced by microorganisms, called natural products, or derivatives thereof. The existing anti-infective agents have become less effective as pathogens evolve resistance to these molecules. Consequently, we need to discover new anti-infectives and also develop methods to diversify/optimise the structure of these molecules if we are to combat emerging antimicrobial resistance (AMR) and provide treatments for future pandemics. In this project, we aim to use state-of-the-art synthetic biology technologies, including CRISPR-Cas9 gene editing and directed evolution, to create new enzymes and pathways that deliver novel antibiotics and new anti-viral agents. Initially we will focus on nonribosomal peptide natural products that show promising antimicrobial activity. We will characterise the key enzymes required for the biosynthesis of these natural products, including non-ribosomal peptide synthetase (NRPS) and various tailoring enzymes that introduce functionality that is essential for activity. We will then use this knowledge to guide engineering of NRPS enzymes that accept alternative substrates and evolve new tailoring enzymes to derivatise and further diversify the antibiotic scaffolds. The new suite of enzymes will be introduced into an optimised Streptomyces super-host strain to create new pathways that can deliver new antibiotics which are urgently required to combat AMR.

Training will be provided in protein engineering, directed evolution, enzyme characterisation (including X-ray crystallography) and enzyme assays. There will also be scope within the project to develop skills in molecular biology and microbiology, including manipulation of Streptomyces bacteria, or alternative host strains. Candidates are not expected to have expertise in all these areas at the outset; above all, scientific curiosity and a desire to work in a multidisciplinary environment are most important. Candidates with a degree in Chemistry, Biochemistry or Biological Sciences and an interest in enzyme catalysis (biocatalysis), biosynthesis (natural products), microbiology or a related science are encouraged to apply. The project will be supervised by Professor Jason Micklefield, in collaboration with Professors Patrick Cai and Anthony Green. 

www.micklefieldlab.chemistry.manchester.ac.uk

www.greenresearchgroup.co.uk

www.cailab.org

Applicants must have obtained or be about to obtain a First or Upper Second class UK honours degree, or the equivalent qualifications gained outside the UK, in an appropriate area of science, engineering or technology.

Applicants interested in this project should make direct contact with the Primary Supervisor to arrange to discuss the project further as soon as possible.

How To Apply

To be considered for this project you MUST submit a formal online application form - full details on how to apply can be found on the BBSRC DTP website www.manchester.ac.uk/bbsrcdtpstudentships

We value your feedback on the quality of our adverts. If you have a comment to make about the overall quality of this advert, or its categorisation then please send us your feedback
Advert information

Type / Role:

Subject Area(s):

Location(s):

PhD tools
 

PhD Alert Created

Job Alert Created

Your PhD alert has been successfully created for this search.

Your job alert has been successfully created for this search.

Ok Ok

PhD Alert Created

Job Alert Created

Your PhD alert has been successfully created for this search.

Your job alert has been successfully created for this search.

Manage your job alerts Manage your job alerts

Account Verification Missing

In order to create multiple job alerts, you must first verify your email address to complete your account creation

Request verification email Request verification email

jobs.ac.uk Account Required

In order to create multiple alerts, you must create a jobs.ac.uk jobseeker account

Create Account Create Account

Alert Creation Failed

Unfortunately, your account is currently blocked. Please login to unblock your account.

Email Address Blocked

We received a delivery failure message when attempting to send you an email and therefore your email address has been blocked. You will not receive job alerts until your email address is unblocked. To do so, please choose from one of the two options below.

Max Alerts Reached

A maximum of 5 Job Alerts can be created against your account. Please remove an existing alert in order to create this new Job Alert

Manage your job alerts Manage your job alerts

Creation Failed

Unfortunately, your alert was not created at this time. Please try again.

Ok Ok

Create PhD Alert

Create Job Alert

When you create this PhD alert we will email you a selection of PhDs matching your criteria.When you create this job alert we will email you a selection of jobs matching your criteria. Our Terms and Conditions and Privacy Policy apply to this service. Any personal data you provide in setting up this alert is processed in accordance with our Privacy Notice

Create PhD Alert

Create Job Alert

When you create this PhD alert we will email you a selection of PhDs matching your criteria.When you create this job alert we will email you a selection of jobs matching your criteria. Our Terms and Conditions and Privacy Policy apply to this service. Any personal data you provide in setting up this alert is processed in accordance with our Privacy Notice

 
 
 
More PhDs from The University of Manchester

Show all PhDs for this organisation …

More PhDs like this
Join in and follow us

Browser Upgrade Recommended

jobs.ac.uk has been optimised for the latest browsers.

For the best user experience, we recommend viewing jobs.ac.uk on one of the following:

Google Chrome Firefox Microsoft Edge