PhD Studentship: Developing Novel Methods to Watch Membrane Proteins Move

We study the architecture and functional dynamics of membrane proteins, especially many medically relevant membrane transport systems. There is increasing evidence that membrane proteins do not act alone, but that they are organised as nano-machineries which function through the concerted action of individual components with high precision and specificity observed in both time and space. We are seeking to unravel the principles underlying the architecture and dynamics of these protein nanomachineries as well as their function. Our experimental approach focuses on the use of magnetic resonance techniques specifically Electron Paramagnetic Resonance (EPR) and Nuclear Magnetic Resonance (NMR) spectroscopy in combination with molecular biological, and biochemical approaches.

This project will study a specific sodium symporter transporter LeuT, a small amino-acid transporter from Aquifex aeolicus, which is a structural homologue of the human neurotransmitter transporters for dopamine, serotonin, norepinephrine and amino butyric acid. These human transporters are implicated in several diseased states including depression, anxiety and attention-deficit hyperactivity disorder. Several prescribed medications target these transporters and illicit street drugs like cocaine or amphetamine interact with them. However, the LeuT transporter is highly dynamic and the development of medically relevant SLC6 inhibitors relies on understanding the distribution of conformational states. Recent structural studies have proposed large scale conformational changes and we aim to probe the functional dynamics of this protein family using a combination of state-of-the-art magnetic resonance techniques as well as techniques to purify and stabilise the protein using novel SMA lipid particles. The successful candidate will receive world class training and become competent in a wide range of biochemical and biophysical techniques working within a team environment using state-of-the-art equipment to study an important family of membrane transporters with societal impact.

The Norwich Research Park (NRP) Biosciences Doctoral Training Programme (DTP) is offering fully-funded studentships for October 2023 entry. The programme offers postgraduates the opportunity to undertake a 4-year PhD research project whilst enhancing professional development and research skills through a comprehensive training programme.

You will join a vibrant community of world-leading researchers.

All NRPDTP CASE students undertake a three to 18-month placement with the non-academic partner during their study. The placement offers experience designed to enhance professional development.

Full support and advice will be provided by our Professional Internship team.

Students with, or expecting to attain, at least an upper second class honours degree, or equivalent, are invited to apply.

This project has been shortlisted for funding by the NRPDTP. Shortlisted applicants will be interviewed either on 16^th or 17^th May 2023. 

Please note that all international awards have been made for our programme for 2023 so we will not be accepting applications from international candidates, as defined by UKRI’s guidance on International Eligibility criteria for UKRI funded studentships - View Website*

Visit our website for further information on eligibility and how to apply: https://biodtp.norwichresearchpark.ac.uk/

Our partners value diverse and inclusive work environments that are positive and supportive. Students are selected for admission without regard to gender, marital or civil partnership status, disability, race, nationality, ethnic origin, religion or belief, sexual orientation, age or social background.

PRIMARY SUPERVISOR: Dr Iain Macaulay

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