|UK Students, EU Students, International Students
|23rd November 2023
|25th January 2024
Supervisors: Peng Wang (Physics)
The project aims to enhance Single Particle Analysis (SPA) software, a vital 3D imaging tool in structural biology, by integrating GPU-enhanced ptychography, an advanced computational microscopy technique, into a worldwide SPA framework. Traditional SPA techniques face limitations in resolving small and complex biological macromolecules. By incorporating ptychography, known for its high phase-sensitivity and applicability in low-dose data scenarios, researchers can achieve higher resolution in cryo-electron microscopy. This integration extends the utility of SPA, allowing researchers to work with diverse imaging modalities, significantly elevate data quality, and push the boundaries of resolution. Through international collaboration with leading experts and cutting-edge resources, we aim to advance structural biology, providing the potential for more precise macromolecular reconstructions and overcoming current challenges.
Single Particle Analysis (SPA) software is a critical tool in the field of structural biology, especially for cryo-electron microscopy (cryo-EM). It plays a pivotal role in extracting structural information from biological macromolecules, enabling the generation of high-resolution 3D reconstructions. However, traditional SPA techniques have primarily relied on conventional transmission electron microscopy (TEM) imaging at high defocus. The low contrast and signal-to-noise ratio in defocused TEM images make it particularly challenging to achieve atomic-level resolution for small, heterogeneous and/or flexible molecules or complexes. Ptychography is an emerging computational microscopy technique for acquiring images with resolution beyond the limits imposed by lenses, which has been applied to high resolution x-ray imaging. Due to its high phase-sensitivity, robustness to low electron dose data and the recovery of the sample wavefunction, it represents a potentially disruptive change in the rapidly growing field of cryo-EM.
The HetSys' training programme is designed to enable students to become high-quality computational scientists who are comfortable working in interdisciplinary environments, have excellent communication skills, and well prepared for a wide range of future careers in areas where there is demonstrable need.
To apply, please click on the 'Apply' button above.
Awards for both UK residents and international applicants pay a stipend to cover maintenance as well as paying the university fees and a research training support. The stipend is at the standard UKRI rate.
For more details visit: https://warwick.ac.uk/fac/sci/hetsys/apply/funding/
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