Wellcome Sanger Institute

Postdoctoral Fellow - Single Cell Analysis of Microenvironmentally-modulated Cancer Drug Resistance Mechanisms

Wellcome Sanger Institute

Do you want to help us improve human health and understand life on Earth? Make your mark by shaping the future to enable or deliver life-changing science to solve some of humanity’s greatest challenges.

About the Role:

We have an exciting opportunity for a Postdoctoral Fellow to be based in the laboratory of Dr. Mathew Garnett for a 3 year fixed term contract.

About Us:

You will be joining a dynamic multi-disciplinary team with expertise in performing systematic perturbation screens in cancer cell lines and patient-derived organoids, and have led the creation of widely used reference cancer therapeutics resources. The project is part of the PERSIST-seq consortium, involving 14 European partner, which aims to use single-cell sequencing to improve our understanding of therapeutic resistance in cancer and create effective strategies to improve cancer treatment.

You will be responsible for:

The tumour microenvironment modulates therapy response and provides ‘niches’ to support drug-tolerant resistor cells that contribute to drug resistance and disease reoccurrence. This includes for example supportive cell-cell interactions, paracrine signalling, the presence of tumour-associated fibroblasts, or creating an immunosuppressive environment. This project involves leveraging the PERSIST-Seq single-cell sequencing platform to mechanistically interrogate the role of the microenvironment in mediating drug resistance and to identify interventions that disrupt these supportive cues to irradicate resistant cells. You will use your knowledge of cell types, cellular transcriptional states, and spatial contexts to develop in vitro tumour organoid culture conditions that recapitulate the microenvironmental cues that support DTP cells. These will be used to test interventions that selectively target drug-tolerant persistor cells to prevent drug resistance.

About You:

You will have experience using advanced in vitro cell culture models such as 3D organoids, primary cells, co-cultures, spheroid, iPSC, or similar cell culture systems. They should have a range of cellular biology skills, including performing Western blotting, cell viability assays, lentivirus generation, CRISPR gene-editing, and FACS. Knowledge of developmental biology and cancer therapeutics are desirable. Ideally, you would have some experience working with single-cell sequencing data and have basic programming skills in R or Python. You should enjoy working as part of a collaborative team in a dynamic multi-disciplinary laboratory. A supportive training environment will be provided to extend and complement your skills.

You will be part of a vibrant international Postdoctoral Fellow Community at the Sanger Institute with a wide programme of transferable skills training and excellent career development opportunities. For more information about being a Postdoctoral Fellow - please visit: https://youtu.be/1-vxm2nBmFA

Essential Skills:

  • PhD in biology, developmental biology, biotechnology, cell biology, or related discipline

  • Experience performing mammalian cell culture

  • Experience performing molecular cloning, lentiviral production, Western blotting, flow cytometry, and viability assays

  • Ability to communicate ideas and results effectively to diverse stakeholders through discussion, talks, and publications.

  • The ability to influence others for positive outcomes through transparent and engaging communication.

  • Ability to constructively resolve differences and conflict.

  • Promote a positive, safe and efficient working environment.

  • Effective and confident decision-making skills.

  • Strategic thinking skills and the ability to embrace change and seek creative solutions to challenges.

  • Introduce leading edge and transformational ideas.

  • Take ownership of work, demonstrating pride, adaptability and accountability for achieving results.

  • Uphold the highest ethical and scientific standards.

  • Proven ability to develop and maintain effective working relationships with wide range of persons of differing levels, abilities and knowledge.

  • Ability to effectively prioritise, multi-task and work independently

  • Detailed orientated, strong organisational and problem-solving skills

  • Commitment to personal development and updating of knowledge and skills

  • Demonstrates inclusivity and respect for all

Application Process:

Please apply with your CV and a Cover letter outlining how you meet the skills set out above.

Working at Wellcome Sanger

Our flexible-hybrid working environment is designed to support a healthy work-life balance. This means you can work flexibly with a combination of working from home, and working from our Campus to allow you to focus on being productive and part of the team while enjoying the benefits of working flexibly.

We aim to attract, recruit, retain and develop talent from the widest possible talent pool, thereby gaining insight and access to different markets to generate a greater impact on the world. We have a supportive culture with the following staff networks, LGBTQ+, Parents and Carers and Race Equity to bring people together to share experiences, offer specific support and development opportunities and raise awareness. The networks are also a place for allies to provide support to others.

We want our people to be whoever they want to be because we believe people who bring their best selves to work, do their best work. That’s why we’re committed to creating a truly inclusive culture at Sanger Institute. We will consider all individuals without discrimination and are committed to creating an inclusive environment for all employees, where everyone can thrive.