Post Doctoral Researcher in Functional Genomics

The overall purpose of the role is to lead an ambitious, BBSRC-funded project which aims to establish a biological resource to enable the in-depth study of transcription factor function in Drosophila by generating and injecting a collection of plasmids to allow the CRISPR-mediated endogenous tagging of all Drosophila transcription factors with an exchangeable epitope.

 

• Collaborating closely with the line manager and other team members on the research project to establish a collection of transgenic Drosophila lines with tagged transcription factors
• Designing transgenesis constructs and help with their molecular cloning and helping to generate transgenic fly strains
• Performing immunostains on different Drosophila tissues and confocal imaging of the samples to analyse transcription factor expression patterns
• Performing genome-wide transcription factor binding site analysis using CUT&RUN sequencing
• Populating and maintaining a database with imaging and binding profile data
• Assisting with the preparation of manuscripts for publication and presenting data at conferences.

• PhD in Genetics, Developmental Biology, Molecular Biology, Biochemistry or equivalent
• Experience with general molecular biology methods, especially molecular cloning
• Experience with the preparation of scientific publications
• Good knowledge of Drosophila biology, eukaryotic gene regulation, CRISPR-mediated transgenesis
• Experience in functional genomics techniques such as ChIP-seq and/or CUT&RUN sequencing and the analysis of functional genomics data
• Experience in immunostaining and confocal microscopy.

As one of the largest employers in Oxford we pride ourselves in the great experience we offer our staff. You'll be joining a friendly, professional environment where every member of staff is recognised as important to the success of Oxford Brookes University.  To find out more about the benefits of working for Oxford Brookes please visit: www.brookes.ac.uk/job-vacancies/working-at-brookes.

 

The University has adopted equality, diversity and inclusion as core values.  We welcome applications from suitably qualified candidates whatever their background, and especially from BAME candidates who are under-represented in our workforce.

 

Please note, due to the volume of applications, we are not able to provide feedback to applicants that are not shortlisted.

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