Research Technician

Job Summary

This post has been created as part of a multidisciplinary project funded by the Wellcome Trust to investigate the neuro-immune-stromal interactions in response to environmental change and how this drives allergic airway inflammation and remodelling.

Environmental factors such as pollutants and pathogens are instigators of injury and contribute to asthma severity, but it is unclear how and why the body’s response to such exogenous stimuli remains aberrant in asthmatics. To investigate this further, the post holder will help develop clinically relevant in vitro systems to model the epithelial mucosal barrier, underlying stromal cells and immune effector cells and assess their response to environmental triggers.

The post holder will also provide a range of technical support to our research programme. This will involve a variety of in vivo and in vitro techniques to evaluate the cells and molecules involved in the development of airway remodelling, as well as in vivo measurement of airway function.

Under the overall direction and supervision of the line manager the post holder will gain experience and apply and extend his/her knowledge of relevant laboratory techniques. The post holder will also be expected to undertake general laboratory duties (https://lloydlab.co.uk).

Duties & Responsibilities

• Develop lung organoid models together with members of the Lloyd team.
• Support ongoing experiments in the lab e.g., genotyping transgenic colonies, measurement of lung function, sampling of the airways, tissue preparation and RNA and DNA preparation.
• Conduct cytokine assays, histological analysis, immunocytochemistry, in situ hybridisation, flow cytometry, in vitro culture of leukocytes and lung structural cells
• Assist other members of the lab with their in vivo experiments.
• General laboratory administrative duties including ordering when needed.

Essential Requirements

• BSc in basic biological sciences or equivalent vocational qualification
• Work independently and be competent in general lab techniques, including Flow cytometry, qPCR, ELISA, Cell culture, IHC/IF, WB, DNA extraction, genotyping, bioinformatics, data analysis.
• Practical experience with in vivo mouse models would be desirable.

Further Information

You will be required to complete an online application for this post.

Should you require any further details on the role please contact: Laura Yates, Research Manager Lloyd Lab -  l.yates@imperial.ac.uk.

For technical issues when applying online please email recruitment@imperial.ac.uk

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