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Research Assistant, MDBS

Queen's University Belfast - Health and Medical - School of Medicine, Dentistry & Biomedical Sciences (MDBS)

Location: Belfast
Salary: £32,024
Hours: Full Time
Contract Type: Permanent
Placed On: 19th February 2024
Closes: 14th March 2024
Job Ref: 24/111637

The School of Medicine, Dentistry & Biomedical Sciences (MDBS) at Queen’s University Belfast, is currently seeking to appoint an exceptional candidate to the post of Research Assistant.

The Microbial Biochemistry and Pathogenesis group of Professor Valvano, in the Wellcome-Wolfson Institute for Experimental Medicine, is looking for an ambitious, highly productive individual with an interest and demonstrated research skills on developing cellular models of bacterial infection in macrophages and lung epithelial cells. The candidate will be involved in ongoing and planned research aiming to understand the molecular and cellular pathogenesis of lung opportunistic Gram-negative bacteria.

The post holder will apply specialized imaging, molecular microbiology, and cellular biology techniques to examine the course of infection in primary immune and non-immune (e.g., macrophages and epithelial) cells.

Applications are invited from individuals with combined expertise in microbiology, microbial genetics, molecular biology, and cell biology, and a strongly demonstrated commitment to research. Examples include recent research experience in cell culture, handling of fastidious Gram-negative pathogenic, single-cell transcriptomics, advanced confocal microscopy cellular imaging, and imaging modelling coupled to tri-dimensional cell image reconstruction techniques.

The successful candidate must have, and your application should clearly demonstrate that you meet the following criteria:

  • A Degree in Microbiology, Immunology, Genetics or Cell Biology
  • Demonstrated experience in culturing primary human macrophages and primary epithelial cells, especially using the air-liquid interface techniques.
  • Recent experience with infection quantification in cells and confocal fluorescent imaging methods using live or fixed samples, relevant to the project.
  • Recent experience with identification by confocal fluorescence microscopy imaging of intracellular compartments/organelles and associated bacteria.
  • Demonstrated experience in in silico imaging modelling and tri-dimensional cell image reconstruction techniques.
  • Recent research experience in state-of-the-art molecular (e.g. cloning using Gibson ligation) and cellular (bacterial infections in eukaryotic cells) microbiology, cell culture, and cellular and molecular imaging techniques including super-resolution microscopy.
  • Prior experience in handling Gram-negative bacterial pathogens relevant to the project.
  • Prior experience in extracting mRNA and processing for single-cell transcriptomic analyses.

Please note the above are not an exhaustive list. For further information about the role including the essential and desirable criteria, please visit our website.

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