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Research Assistants

King's College London - Faculty of Life Sciences & Medicine

Location: London
Salary: £37,332 to £39,980 per annum, including London Weighting Allowance.
Hours: Full Time
Contract Type: Fixed-Term/Contract
Placed On: 27th February 2024
Closes: 24th March 2024
Job Ref: 084930
 

Location: Guy's Campus

Contact details: Professor Shanta Persaud. shanta.persaud@kcl.ac.uk

Job description

Applications are invited for four-year Research Assistant positions working on a project funded by the Steve Morgan Foundation entitled ‘Towards translation: improving the functional survival of stem cell-derived beta cells’. The successful applicant will work under the supervision of Prof. Shanta Persaud, Dr. Aileen King and Prof. Peter Jones in the Diabetes Theme within the School of Cardiovascular and Metabolic Medicine & Sciences at King’s College London. The project will use information from our spatial transcriptomic analysis of human fetal pancreas development to improve the generation of functional stem cell-derived beta cells, and then apply our expertise in beta cell/islet biology to ensure the optimal performance of stem cell-derived islets in vitro and in vivo. This research assistant post will provide support to a post-doctoral researcher, appointed to start at the same time. 

Research Assistant 1

The successful candidate will have a BSc, MSc or PhD in a relevant biomedical sciences subject and be familiar with human stem cell (ES or iPS) differentiation protocols. The main responsibilities will be supporting the post-doctoral researcher with human stem cell culture and differentiation; assessing the potential of Schwann cells and their secretory products to improve stem cell-derived beta cell maturation; configuring stem cell-derived beta cells into functional islet-like structures; and scRNA analysis of stem cell-derived islets. This research assistant post will be held within a vibrant islet biology research community at the Guy’s campus of King’s College London.

Research Assistant 2 

The successful candidate will have a BSc, MSc or PhD in a relevant biomedical sciences subject and be familiar with islet biology and in vivo models of type 1 diabetes. The main responsibilities will be supporting the post-doctoral researcher with determining the effects of mesenchymal stromal cell secretory products and extracellular matrix on stem cell-derived islet function;  nanoencapsulating stem cell-derived islets to deliver biotherapeutics; and evaluating the effects of the best performing stem cell-derived islets in diabetic mice in vivo. This research assistant post will be held within a vibrant islet biology research community at the Guy’s campus of King’s College London

Specific queries relating to the post can be directed to shanta.persaud@kcl.ac.uk.

This post will be offered on a fixed-term contract, with proposed employment dates of 01/06/2024-31/05/2028.

This is a full-time post - 100% full time equivalent.

Skills, knowledge, and experience

Research Assistant 1

Essential criteria

  • BSc, MSc or PhD in a relevant biomedical sciences subject.
  • Research experience in a molecular cell biology discipline.
  • Familiarity with human stem cell culture and/or differentiation protocols
  • Cell culture experience.
  • Familiarity with general molecular cell biology techniques, such as qRT-PCR, scRNAseq, immunohistochemistry, immunoassays, proteomics.

Desirable criteria

  • Experience with human stem cell differentiation.
  • Experience with islets of Langerhans.

Research Assistant 2

Essential criteria

  • BSc, MSc or PhD in a relevant biomedical sciences subject. 
  • Research experience in a molecular cell biology discipline. 
  • Cell culture experience. 
  • Familiarity with general molecular cell biology techniques, such as qRT-PCR, scRNAseq, immunohistochemistry, immunoassays. 

Desirable criteria

  • Experience with islets of Langerhans. 
  • Experience of using mouse models for diabetes or metabolic studies.

Further information

Interviews will be held in March. Candidates will be notified at least 5 days prior to the interview if they have been shortlisted. We would like the successful candidate to start on 01 June 2024.

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