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Research Technician

Imperial College London

Location: London
Salary: £30,273 to £33,180 per annum
Hours: Full Time
Contract Type: Fixed-Term/Contract
Placed On: 5th October 2021
Closes: 28th October 2021
Job Ref: MED02732
 

Location: South Kensington Campus

Job Summary

This post is specifically to assist in carrying out experiments related to Dr Mohamed Shamji’s research focused on the investigation of immunological mechanisms of tolerance induction following AIT in allergic diseases. This involves assessing the effect of AIT on innate (group 2 innate lymphoid cells, dendritic cells and epithelial cells) and adaptive (T and B cells) responses. Moreover, his group aims to identify novel biomarkers of clinical outcome following a successful allergen immunotherapy.

The post-holder will provide technical support to Dr. Shamji’s group within Allergy and Clinical Immunology in the division of Inflammation, Repair & Development. The post-holder will be involved in multiple projects, including supporting internal clinical trial running within the group. Experience in running biomarker assays in the context of allergy research and B/T cell phenotyping for next generation sequencing techniques and/or the analysis of next generation sequencing data sets would be advantageous. The ability to work independently and as part of a team, excellent communication, organizational and problem-solving skills are essential. Under the overall direction and supervision of the line manager, you will apply and extend your knowledge of relevant laboratory techniques.  In addition to providing technical support, the duties include laboratory administrative responsibilities.

Duties and responsibilities

Under the direction of the line manager, you will be involved in:

  • Planning and executing experiments using the following techniques:
    • Cell separation
    • Cell Culture
    • FACS analysis
    • ELISA
    • DNA/RNA extraction and quantification
    • PCR
    • Library preparation for Next Generation Sequencing (NGS)
  • The collection, preparation, processing, and storage of samples
  • Isolation, culturing, and experimentation with primary cells
  • Maintenance of cell line cultures and purifying primary cells
  • General laboratory administrative duties including ordering
  • Maintaining stocks of cell lines, virus and routine supplies

Essential requirements

  • You should hold a Master’s degree (MSc or MRes) in Life Sciences or equivalent
  • Experience in laboratory-based research
  • Practical experience with working with peripheral blood
  • In depth knowledge of allergy and immunology
  • Experience in collection, preparation, processing and/or storage of samples
  • Experience with data analysis for flow cytometry (viSNE and FlowSOM)

Further Information

The post is a Full Time & Fixed Term role for 12 months with possibility of extension, based at the South Kensington Campus, London.

Should you require any further details on the role please contact: DR Mohamed Shamji – m.shamji@imperial.ac.uk.

Closing date: 28 Oct 2021

To apply, visit www.imperial.ac.uk/jobs and search by the job reference MED02732

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