Back to search results

Research Associate

University of Bristol - Faculty of Life Sciences

Location: Bristol
Salary: £34,304 to £38,587 per annum
Hours: Full Time
Contract Type: Permanent
Placed On: 27th April 2022
Closes: 24th May 2022
Job Ref: ACAD106039

The role

A postdoctoral research position in de novo membrane protein design is available for 36 months in the School of Biochemistry at the University of Bristol, supported by the BBSRC Grant BB/W003449/1, Creating and comprehending the circuitry of life: precise biomolecular design of multi-centre redox enzymes for a synthetic metabolism.  The project is a collaboration between Dr Ross Anderson, Prof Adrian Mulholland, Dr Tom Oliver, Dr Paul Curnow (University of Bristol), Prof Julea Butt (UEA), Dr Bruce Lichtenstein (Portsmouth) and Dr Amandine Marechal (UCL).

The multidisciplinary project aims to construct single and multi-centre redox and light harvesting proteins and enzymes, photocatalytic de novo enzymes, and artificial respiratory complexes, providing an unprecedented framework to better understand and exploit the exceptional properties of the natural electron and energy conducting machinery.

The postdoctoral research associate will work on designing cofactor-binding membrane proteins and assemblies for electron transfer, light harvesting and catalysis. The ambition is to establish a suite of modular designs that can interact with soluble enzymes to create more complex binary and chain-containing architectures. They will also work closely with other researchers on the project, combining expertise in membrane protein design, biomolecular simulation, chemical synthesis and spectroscopy towards the construction of artificial respiratory complexes and functional components for bionanotechnology and bioengineering.

Further details of the BBSRC grant can be found here: https://circuitsoflife.co.uk. More information on Paul Curnow’s research can be found at https://tinyurl.com/pcurnow and Ross Anderson’s group can be found at: https://theandersonlab.com

What will you be doing?

You will use computational methods, specifically the membrane extension of Rosetta and Molecular Dynamics, to design a range of single and multi-centre membrane redox proteins that can be employed in long range electron and energy transfer, catalysis and broadband light harvesting. You will then use standard molecular biology techniques to express and purify yourdesigns in recombinant cell systems. This will likely involve the screening of different solubilising detergents to obtain stable, soluble membrane proteins in vitro. An array of biophysical techniques will then be employed to verify and study structure and function (e.g. visible/CD/fluorescence/NMR spectroscopies, protein electrochemistry, stopped flow spectrophotometry, X-ray crystallography, cryo-EM microscopy, mass spectrometry).

You should apply if

This position would best suit a talented and motivated early career researcher with a PhD in Biochemistry or Chemistry. Prior experience in computational membrane protein design (RosettaMP, Molecular Dynamics) would be an advantage as would knowledge of, or experience in, bioenergetics. You should be able to demonstrate skills in molecular biology, protein expression/purification, and structural and biophysical protein analysis. Preferred candidates are likely to be familiar with applying these methods to integral membrane proteins, and relevant skill sets – for example, experience in detergent screening methods – would be advantageous. You should also have the ability to communicate complex information clearly and accurately in English, both in written and oral forms; and the ability to work both independently and as part of a research team.

For informal enquiries please contact Dr Paul Curnow, p.curnow@bristol.ac.uk.

We welcome applications from all members of our community and are particularly encouraging those from diverse groups, such as members of the LGBT+ and BAME communities, to join us.

We value your feedback on the quality of our adverts. If you have a comment to make about the overall quality of this advert, or its categorisation then please send us your feedback
Advert information

Type / Role:

Subject Area(s):

Location(s):

Job tools
 

PhD Alert Created

Job Alert Created

Your PhD alert has been successfully created for this search.

Your job alert has been successfully created for this search.

Ok Ok

PhD Alert Created

Job Alert Created

Your PhD alert has been successfully created for this search.

Your job alert has been successfully created for this search.

Manage your job alerts Manage your job alerts

Account Verification Missing

In order to create multiple job alerts, you must first verify your email address to complete your account creation

Request verification email Request verification email

jobs.ac.uk Account Required

In order to create multiple alerts, you must create a jobs.ac.uk jobseeker account

Create Account Create Account

Alert Creation Failed

Unfortunately, your account is currently blocked. Please login to unblock your account.

Email Address Blocked

We received a delivery failure message when attempting to send you an email and therefore your email address has been blocked. You will not receive job alerts until your email address is unblocked. To do so, please choose from one of the two options below.

Max Alerts Reached

A maximum of 5 Job Alerts can be created against your account. Please remove an existing alert in order to create this new Job Alert

Manage your job alerts Manage your job alerts

Creation Failed

Unfortunately, your alert was not created at this time. Please try again.

Ok Ok

Create PhD Alert

Create Job Alert

When you create this PhD alert we will email you a selection of PhDs matching your criteria.When you create this job alert we will email you a selection of jobs matching your criteria. Our Terms and Conditions and Privacy Policy apply to this service. Any personal data you provide in setting up this alert is processed in accordance with our Privacy Notice

Create PhD Alert

Create Job Alert

When you create this PhD alert we will email you a selection of PhDs matching your criteria.When you create this job alert we will email you a selection of jobs matching your criteria. Our Terms and Conditions and Privacy Policy apply to this service. Any personal data you provide in setting up this alert is processed in accordance with our Privacy Notice

 
 
More jobs from University of Bristol

Show all jobs for this employer …

More jobs like this
Join in and follow us

Browser Upgrade Recommended

jobs.ac.uk has been optimised for the latest browsers.

For the best user experience, we recommend viewing jobs.ac.uk on one of the following:

Google Chrome Firefox Microsoft Edge