Back to search results

PDRA - In Situ Structural Biochemistry (10266)

Rosalind Franklin Institute

Location: Harwell
Salary: £33,500 to £41,000 per annum
Hours: Full Time
Contract Type: Fixed-Term/Contract
Placed On: 7th March 2023
Closes: 10th April 2023
Job Ref: 10266

PDRA - In Situ Structural Biochemistry (10266) - Rosalind Franklin Institute (rfi.ac.uk) 

We are interested in understanding the cellular basis underlying biosynthesis of the glycosaminoglycan polysaccharide heparan sulfate (HS), a complex heterogenous molecule involved in vital physiological processes. The biosynthesis of HS is known to take place in the Golgi compartment of cells. Many of the enzymes responsible for HS construction are now characterized, but a unified picture of how they act together in cells remains lacking. 

Cryo-electron tomography, coupled with focused ion beam milling, offers a powerful route towards in situ structural characterization (e.g., Berger et al, Nat Comms, 2023). However, smaller proteins, such as the enzymes involved in HS construction, are still difficult to identify within information dense tomograms. There is thus a pressing need to develop methods to tag proteins of interest in native contexts, so they can be recognised even within the crowded intracellular environment. 

Your primary task will be to develop new methods to modify proteins of interest, to facilitate their detection by cryo-electron tomography and associated techniques. Working with researchers across the Franklin, you will utilize chemical, biochemical and/or genetic methods to add tags such as fluorophores or heavy atoms to proteins in a site selective fashion. You will develop strategies to apply your tagging methods within intracellular milieu and use multiple imaging modalities to verify successful incorporation and detection. Although early stages of the project will focus on in vitro models, your ultimate targets will be intracellular Golgi enzymes involved in heparan sulfate construction.  

This post would suit an ambitious candidate with expertise in protein (bio)chemistry, who wishes to apply their knowledge to complex cellular systems. It is envisioned the successful candidate would bridge existing interests in the Franklin regarding both protein modification and complex multimodal imaging. You will be afforded training on high end electron imaging methods, with access to cutting edge and beyond state-of-the-art EM platforms. 

Before submitting your application, please ensure you read the Job Information Pack for full details of this role by clicking on the attachment at the bottom of our job advert:

PDRA - In Situ Structural Biochemistry (10266) - Rosalind Franklin Institute (rfi.ac.uk) 

About us: The Rosalind Franklin Institute is a new national Research Centre, funded by the UK government through UK Research and Innovation, dedicated to bringing about transformative changes in life science through interdisciplinary research and technology.  

Location: The Franklin is based in Harwell Campus, UK’s innovation hub and well-established as one of the leading science and innovation campuses in Europe.   

Closing date: Monday 10th April 2023

Interviews will be held w/c 24th April 2023.

Area: Scientific

Period of appointment: 3 years fixed term in the first instance

We value your feedback on the quality of our adverts. If you have a comment to make about the overall quality of this advert, or its categorisation then please send us your feedback
Advert information

Type / Role:

Subject Area(s):

Location(s):

Job tools
 

PhD Alert Created

Job Alert Created

Your PhD alert has been successfully created for this search.

Your job alert has been successfully created for this search.

Ok Ok

PhD Alert Created

Job Alert Created

Your PhD alert has been successfully created for this search.

Your job alert has been successfully created for this search.

Manage your job alerts Manage your job alerts

Account Verification Missing

In order to create multiple job alerts, you must first verify your email address to complete your account creation

Request verification email Request verification email

jobs.ac.uk Account Required

In order to create multiple alerts, you must create a jobs.ac.uk jobseeker account

Create Account Create Account

Alert Creation Failed

Unfortunately, your account is currently blocked. Please login to unblock your account.

Email Address Blocked

We received a delivery failure message when attempting to send you an email and therefore your email address has been blocked. You will not receive job alerts until your email address is unblocked. To do so, please choose from one of the two options below.

Max Alerts Reached

A maximum of 5 Job Alerts can be created against your account. Please remove an existing alert in order to create this new Job Alert

Manage your job alerts Manage your job alerts

Creation Failed

Unfortunately, your alert was not created at this time. Please try again.

Ok Ok

Create PhD Alert

Create Job Alert

When you create this PhD alert we will email you a selection of PhDs matching your criteria.When you create this job alert we will email you a selection of jobs matching your criteria. Our Terms and Conditions and Privacy Policy apply to this service. Any personal data you provide in setting up this alert is processed in accordance with our Privacy Notice

Create PhD Alert

Create Job Alert

When you create this PhD alert we will email you a selection of PhDs matching your criteria.When you create this job alert we will email you a selection of jobs matching your criteria. Our Terms and Conditions and Privacy Policy apply to this service. Any personal data you provide in setting up this alert is processed in accordance with our Privacy Notice

 
 
More jobs from Rosalind Franklin Institute

Show all jobs for this employer …

More jobs like this
Join in and follow us

Browser Upgrade Recommended

jobs.ac.uk has been optimised for the latest browsers.

For the best user experience, we recommend viewing jobs.ac.uk on one of the following:

Google Chrome Firefox Microsoft Edge