Research Associate / Senior Research Associate

Salary:

£35,333 - £39,745 per annum (Grade I) 

£39,745 - £44,737 per annum (Grade J)

The role

The Research Associate will work on an MRC Impact Acceleration award titled ‘ Erythroid lines as novel and disruptive therapeutic platforms’. We have created novel erythroid lines that recapitulate erythropoiesis, and which provide a sustainable source of RBCs. The lines can be efficiently modified to alter and remove, or express ectopic genes making them potentially valuable and disruptive tools for development and application of wide range of novel therapeutics, and as diagnostic reagents. However, to realise such applications the lines need additional optimisation to further increase resultant RBC yields. The appointed Research Associate will work in Prof Jan Frayne’s lab, alongside a team working on different aspects of erythropoiesis and its regulation in health and disease, to trial a range of reagents and approaches to optimise the lines. The project is in collaboration with a new University spin-out company based in Bristol who have an interest in the lines, and the appointed Research Associate will also work with the companies team, who will provide additional support.

What will you be doing?

The post holder will carry out extensive cell culture, differentiation and manipulation, trialling a range of specifically selected reagents/drugs and modifications to the culture system, along with targeted genetic modifications, to optimise performance. Cells will be analysed throughout culture using a range of techniques including flow cytometry, morphological analysis, qPCR, western blot to monitor efficiency of differentiation and enucleation.

You should apply if

The applicant requires extensive experience of cell culture, preferably of primary cells and analysis of cell differentiation and behaviour, along with multiplex flow cytometry, qPCR, lentiviral transduction of mammalian cells and standard cell biology and molecular biology techniques.

Additional information

For informal enquiries please contact Professor Jan Frayne (Jan.Frayne@bristol.ac.uk). 

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