Rosalind Franklin Institute

Senior Scientist in Automation for CryoET Workflows (10310)

Rosalind Franklin Institute

We are currently seeking a highly motivated Senior Scientist to join our Structural Biology theme at the Rosalind Franklin Institute. Building on recent work demonstrating the use of focused ion beams (FIB) and scanning electron microscopes (SEM) to image cells and tissues (DOIs: 10.1038/s41467-023-36372-9; 10.1101/2022.09.21.508877), we are looking to recruit a computational expert to develop and apply automation to cryogenic workflows for tissues, including the automation of FIB/SEM and lift-out, multimodal correlation and tools for data annotation and processing of cryo-electron microscopy (cryoET) data.

This core funded post builds on recent success in obtaining funding for imaging tissues across scale. The post is aimed at individuals keen to bring a combination of computer science, mathematics, and material science to address questions that bridge molecular-scale imaging with high throughput and on a grand scale. The ideal candidate would have a firm grasp on developing algorithms in python, C++ or other coding languages, and who would be able to apply this to routines on advances electron microscopes.

Working with members of the core facility, the structural biology and artificial intelligence (AI) themes, and research groups at the Franklin, you will develop novel methods that enable automation of in situ cryo ET workflows, enable on-the-fly imaging and plasma focused ion beam milling of samples. This will enable imaging high volumes of lamella of multicellular specimens, implemented alongside efforts to streamline high-precision targeting in 3D and structure determination.

Role Overview

Current in-situ structural biology workflows for cryoET in tissue are laborious, requiring individuals to remain at the side of the microscope. This is due to these capabilities being in their infancy, with critical parameters being established and targeting in bulk materials requiring methods able to recognise and identify regions of interest. At the Franklin, we are establishing workflows capable of investigating multiple tissue samples per day and generating sections able to be thinned to electron transparency. You will develop this working closely with researchers at the Franklin enabling the examination of disease-relevant observations in-situ using multi-modal imaging to streamline the targeting of specific regions of interest, and optimising protocols for ion beam milling.

Before submitting your application, please ensure you read the Job Information Pack for full details of this role by clicking on the attachment.

About us: The Rosalind Franklin Institute is a new national Research Centre, funded by the UK government through UK Research and Innovation, dedicated to bringing about transformative changes in life science through interdisciplinary research and technology.

Location: The Franklin is based in Harwell Campus, UK’s innovation hub and well-established as one of the leading science and innovation campuses in Europe. 

The Franklin’s underlying aim is to produce the best science for research today, and this means resolutely embracing a diverse team, who have a wide range of experiences, skills and knowledge to push forward on the innovative work our institution delivers. Both our work and our institution are better for it. For further information, view our Equality, Diversity and Inclusion Policy

At the Rosalind Franklin Institute we welcome applications from all around the world!

To be considered for this role, please send a CV and cover letter through explaining why you think you are the right person for this job. Please including information about your education and work experience.

Closing date for applications is 23:59 on Sunday 21st January 2024.

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