Research Assistant (Jackson Group) (Fixed Term)

Applications are invited for a full-time Research Assistant in the laboratory of Professor Steve Jackson (www.stevejacksonlab.org) based in the internationally renowned Cancer Research UK Cambridge Institute www.cruk.cam.ac.uk, located on the Cambridge Biomedical Campus, Cambridge, UK. The successful candidate will carry out cell and molecular biology work, assisting with on-going projects in the lab to characterise the cell biology and mechanisms of established and new DNA repair pathways and components. Core duties will involve stable cell line generation, knockout cell line generation using CRISPR/Cas9 technology, routine tissue culture, cloning and other molecular work, as well as biochemical studies, including protein purification and mass spectrometry.

Other responsibilities will include preparation of reagents, maintenance of lab stocks and assist in the day-to-day running of the laboratory.

Preferred skills and experience:

• Degree or equivalent in life sciences
• Strong previous experience of working in a molecular cell biology research laboratory required, ideally with some background in the DNA repair arena
• Mammalian tissue culture, significant experience required (including transfection, siRNA work and stable cell line generation)
• Previous significant experience in molecular biology techniques: cloning, PCR, mutagenesis, western blotting, DNA and RNA isolation and protein analysis
• Experience in CRISPR genome editing and/or CRISPR genetic screens desirable
• Experience in biochemistry, such as protein purification, biochemical assays, and proteomic analysis including mass spectrometry desirable
• FACs and/or fluorescence microscopy experience desirable
• High attention to detail and good record keeping
• Good communication and organisational skills
• Reliability and enthusiasm

Starting date: 1^st March 2023 or as soon as possible after this date.

Fixed-term: The funds for this post are available for 2 years in the first instance.

Once an offer of employment has been accepted, the successful candidate will be required to undergo a security check.

To apply online for this vacancy and to view further information about the role, please visit: www.jobs.cam.ac.uk/job/39352.

Please ensure that you upload a covering letter and CV in the Upload section of the online application. The covering letter should outline how you match the criteria for the post and why you are applying for this role.

If you have any queries relating to this role, or the application process, please contact us at: mailbox-hr@cruk.cam.ac.uk

Please quote reference SW35277 on your application and in any correspondence about this vacancy.

The University actively supports equality, diversity and inclusion and encourages applications from all sections of society.

The University has a responsibility to ensure that all employees are eligible to live and work in the UK.

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